Investigating the Influence of Pre-Donation Haematological Parameters on Haematocrit and the Haemoglobin Content in Packed Red Blood Cell Units: A Single Centre Study

Abstract

Objective: This study aimed to evaluate the impact of pre-donation hematological parameters, specifically hemoglobin and hematocrit levels, on the quality of packed red blood cell (PRBC) units after 21 days of storage.

Methodology: A cross-sectional, analytical study was conducted at the Armed Forces Institute of Transfusion, Rawalpindi from February 2024 to July 2024, involving 384 voluntary whole blood donors who met specific inclusion criteria. Pre-donation hematological parameters were measured using an automated hematology analyzer. PRBC units were processed according to standard protocols and evaluated on Day 21 for volume, hematocrit, and total hemoglobin content. Data analysis was performed using SPSS software, applying descriptive statistics, Pearson correlation, and multiple regression analysis to assess the relationship between pre-donation variables and PRBC quality.

Results: At Day 21, PRBC units exhibited significant changes compared to initial post-donation values. Pre-donation hemoglobin and hematocrit levels showed weak correlations with Day 21 PRBC hemoglobin content and hematocrit levels. The integrity of PRBC units appeared more influenced by storage conditions than donor-specific hematological parameters. While hemoglobin content remained relatively stable, hematocrit levels showed variability, indicating that additional biochemical and environmental factors during storage play a critical role in maintaining PRBC quality.

Conclusion: Pre-donation hematological parameters have a limited role in predicting PRBC quality after 21 days of storage. Factors such as donor conditions and processing parameters may significantly influence PRBC integrity, highlighting the need for comprehensive donor screening and optimized storage practices.