SYBR Green PCR is Cost-Effective for Detecting Genetic Abnormalities in Acute Myeloid Leukemia

Authors

  • Mohammad Abdul Naeem
  • Farhat Ijaz CMH Lahore Medical college and Institute of Dentistry Lahore
  • Mohammad Amir
  • Rana Khurram Aftab

Keywords:

SYBR Green based RT-PCR, acute myeloid leukemia

Abstract

Objective: To determine whether SYBR Green PCR is cost-effective for detecting genetic abnormalities in acute myeloid Leukemia or not.

Methodology: This cross-sectional study was conducted at Department of Pathology-CMH Lahore medical college and institute of dentistry from October 2023- March 2024. DNA samples from 70 adults diagnosed as AML at various hospitals were ran on RT-PCR using dye and probe-based methods for detection of genetic abnormalities in acute myeloid leukemia (AML). Data was analyzed by SPSS 23 and presented in frequencies and percentages.

Results: In a total of 70 patients, twenty-five (17.5%) had defining genetic abnormalities. Fusions were detected in 19 (13.3%) patients while three rearrangements (2.1%) and three mutations (2.1%) were detected. The probe-based RT-PCR matched results in all patients.

Conclusion: SYBR Green PCR is very cost-effective for detecting genetic abnormalities in acute myeloid leukemia.

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Published

2025-01-22

Issue

Vol. 4 No. 2 (2024): July-Dec

Section

Original Article

License

Copyright (c) 2025 Journal of Haematology and Stem Cell Research

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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