Mutations at exons 11 and 17 of c-kit Gene in Patients of Acute Myeloid Leukaemia

Abstract

Objective: To determine the frequency of mutations at exons 11 and 17 of c-kit gene in patients of Acute Myeloid Leukaemia.

Methodology: It was descriptive study and conducted in departments of Hematology and Human Genetics and Molecular Biology, University of Health Sciences, Lahore. Total 80 newly diagnosed patients of AML from different hospitals of Lahore were included in the study The study included both peripheral blood and bone marrow aspirate slides of 80 newly diagnosed patients of AML. Each sample was amplified for exons 11 and 17 of c-kit gene using specific primers by direct DNA sequencing.

Results: Among 80 patients, 67.5% (n = 54) were males and 32.5% (n = 26) were females with a median age of 38 years (range 12-85 years). Our DNA sequencing results indicated that none of the patient had been observed to have mutation in both exons of c-kit gene. Only a polymorphism was identified in exon 11. So, it is concluded that our screened AML population was negative for these two exons of c-kit gene.

Conclusion: The study concluded that our selected AML patients were negative for these two exons but SNP was found in exon 11 of c-kit gene.